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Fig. 6 | BMC Plant Biology

Fig. 6

From: California TRV-based VIGS vectors mediate gene silencing at elevated temperatures but with greater growth stunting

Fig. 6

TRV California vectors induce PDS silencing at different growth temperatures in N. attenuata. A Plants inoculated with pTRV-RNA1/pTRV-RNA2:PDS (PDS) expressed PDS silencing bleaching phenotype at 22 °C, 28 °C and 30 °C conditions. Plants inoculated with the empty vector control (EV) pTRV-RNA1 + pTRV-RNA2:EV (EV) did not have the bleaching phenotype, but displayed viral symptoms in the inoculated leaves at the different temperatures. B Relative PDS transcript abundance in pTRV-RNA1/pTRV-RNA2:PDS (PDS) and pTRV-RNA1 + pTRV-RNA2:EV (EV) inoculated plants grown at 22 °C, 28 °C and 30 °C. The strongest PDS silencing was observed in plants maintained at 28 °C (both with respect to bleaching phenotype and relative PDS transcript abundance). The data of panel B are shown as mean values ±SD; F-test, ** P < 0.01. Photographs of the inoculated plants and the samples for RNA isolation were taken at 13 dpi (days to post inoculation)

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BMC Plant Biology

ISSN: 1471-2229

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