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Fig. 1 | BMC Plant Biology

Fig. 1

From: California TRV-based VIGS vectors mediate gene silencing at elevated temperatures but with greater growth stunting

Fig. 1

Isolation of the full length cDNA of the TRV California genomes from infected N. attenuata. A and B Inoculated plants show the viral symptoms e.g. mottling, localized necrotic lesions, chlorotic spots on the leaves and leaf-deformities in the TRV California inoculated plants. C No such symptoms appeared in the control plants, where only buffer was inoculated. Photographs were taken at 15 dpi. The yellow arrows indicate the virus symptoms on leaves. D and E 1% agarose gel images of PCR amplification of cDNA from full length RNA1 (D, lane RNA1, size ~ 6.6 kb) and RNA2 (E, lane RNA2, ~ 3.5 kb) with TRV California genome specific primers. Lanes ‘L’ represent the 1 kb DNA ladder. The gel images are cropped

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