Fig. 2

Quantity and distribution of D-bodies and AGO1. Localization of Hyl1-YFP and U2 snRNA in protoplasts (A-C) and CDPs cultured for 120 h (D-F) by confocal microscopy. (A-C) Dicer bodies (DB) and Cajal bodies (CB) occur separately at the periphery of the nucleolus (Nu) in protoplasts. In the CDP cells cultured for 120 h, two DBs are in the nucleus (N). Of these, 1 is strongly associated with CB. In C, F dotted line indicate nucleus (G). Percentage of isolated protoplasts (0 h) and CDPs cultured for 24 h, 72 h, and 120 h (dividing cells) with DBs; error bars represent standard deviation. (H) Percentage of DBs associated with nuclear structures in mesophilic cells and subsequent stages of their dedifferentiation (Nu-nucleolus, CB-Cajal body, NE-nuclear envelope). Localization of AGO1 in mesophyll cells (I), isolated protoplasts (J), and CDPs cultured for 24 h (K), 72 h (L) and 120 h (M) under a confocal microscope. Dotted line – nucleus, continuous line – cell, N - nucleus, arrow – clusters of AGO1 in the cytoplasm. Bar 5 μm. The relative fluorescence intensity of AGO1 in the cytoplasm (N) and nucleus (O) in mesophyll cells and during dedifferentiation