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Table 3 Material information table

From: Genetic characteristics and ploidy trigger the high inducibility of double haploid (DH) inducer in Brassica napus

Material name

Ploidy

Source of material

Remarks

Wax or waxless

Y3380

Octoploid (2n = 8× ≈ 76, AAAACCCC)

Provided by Chengdu Academy of Agriculture and Forestry Sciences

Related to DH induction line

wax

Y3560

HZ23

HZ28

3380–1

Mixed ploidy (2n = 4\6\8×)

3560–1 ~ 3560–5

3850

Hexaploid (2n = 6× ≈ 58, AAAACC)

3114

Tetraploid (2n = 4× = 38, AACC)

3128

DW39

3560–6

P3–2

4417

T194

Triploid (2n = 3× ≈ 29, AAC)

HZ1

Hexaploid (2n = 6× ≈ 57, AABBCC)

Provided by Huazhong Agricultural University

Not related to DH induction line

HZ2

HZ4

HZ24

Tetraploid (2n = 4× ≈ 38, AACC)

Provided by Chengdu Academy of Agriculture and Forestry Sciences

3737

T195

Triploid (2n = 3× ≈ 29, AAC)

WlA

pol Cytoplasmic sterile line, Tetraploid (2n = 4× = 38, AACC)

waxless

  1. The materials include octoploid rapeseed: doubled haploid induction lines Y3380 (2n = 8× ≈ 76, AAAACCCC) and Y3560 (2n = 8× ≈ 76, AAAACCCC), HZ23 (2n = 8× ≈ 76, AAAACCCC) and HZ28 (2n = 8× ≈ 76, AAAACCCC). HZ23 is the selfed progeny of Y3560, and HZ28 is the doubled F1 generation of hybrid Brassica napus 908 (2n = 8× ≈ 76). Mixed ploidy: 3380 and 3560 (3560–1 ~ 3560–5, derived from the self-separating progeny of Y3560). Hexaploid rapeseed: 3850 (2n = 6× ≈ 58, AAAACC), HZ1 (2n = 6× = 54, AABBCC), HZ2 (2n = 6× = 54, BBCCAA) and HZ4 (2n = 6× ≈ 58, AAAACC). Among them, 3850 is the self-separated offspring of Y3380, and HZ4 is the artificially doubled offspring of B0486 (2n = 4× = 38, AACC, Brassica napus) × Ya’an Huang (YH, 2n = 2× = 20)) F1 generation. HZ1 (AABBCC) and HZ2 (BBCCAA) are provided by Huazhong Agricultural University. Tetraploid Brassica napus: HZ24, 3114, 3128, DW39, 3560–6, P3–2, 3737, 4417 and W1A. HZ24 (2n = 4× = 38, AACC) ordinary Brassica napus; 3114 (2n = 4× = 38, AACC) and 3128 (2n = 4× = 38, AACC) are Y3380 self-separated Brassica napus (2n = 4× = 38, AACC); DW39 and 3560–6 (2n = 4× = 38, AACC) are self-separating offspring of Y3560; P3–2 (AACC, 2n = 4 × = 38) is the core parent material created by Y3380 and Y3560; 3737 is a common Brassica napus (2n = 4× = 38, AACC); 4417 (2n = 4× = 38, AACC) is a Y3380 × Y3560 separated tetraploid offspring; WlA (2n = 4× = 38, AACC) is a waxless polima cytoplasmic sterile line, used as an induction or hybrid tester species. T194 (2n = 3× = 29) is a triploid rapeseed (F1 generation) synthesized between species (P3–2 (2n = 4× = 38, Brassica napus) × HZ32 (2n = 2× = 20, Brassica rape)) and related to the rapeseed DH induction line. T195 (2n = 3× = 29) is a triploid rape (F1 generation) that is interspecies (ZS11 (2n = 4× = 38, Brassica napus) × HZ32 (2n = 2× = 20, Brassica rape)) and has nothing to do with the DH induction lines