Table 5 Differentially expressed miRNAs in “4001AB”, “4006AB”, “6251AB” and “6284AB” libraries and their candidate targets by sRNA sequencing and transcriptome analysis in Brassica napus

4001

bna-novel_4-3p

8.2

0.4

1.90

BnaC01g31810D,BnaA07g24350D,BnaC02g41520D,BnaA08g22150D,BnaA04g13020D,BnaA07g18960D,BnaA09g38810D,BnaC03g26470D,BnaC02g03200D,BnaA04g00990D,BnaA03g41600D,BnaA05g07140D,BnaA08g19190D

bna-miR390a/b/c

26.8

11.1

1.32

BnaC03g02760D,BnaC01g03570D,BnaA05g28390D,BnaC03g53360D,BnaC01g22410D

bna-novel_31-5p

10.7

55.2

−2.16

BnaC01g21190D,BnaA01g17940D,BnaC03g13660D,BnaA03g10950D,BnaA09g00170D,BnaA10g02710D,BnaC01g22250D,BnaA03g38770D,BnaA02g29970D,BnaA02g18470D

bna-novel_34-5p

0.6

624.0

−8.16

BnaA09g14240D,BnaA05g30840D,BnaA01g24360D,BnaA07g34690D,BnaA03g14400D,BnaA05g09000D,BnaC01g09790D,BnaA08g26410D

bna-novel_1-3p

0.1

8.1

−2.64

–

bna-miR158-3p

554.7

935.8

−0.74

BnaA05g08940D,BnaA04g29200D

4006

bna-novel_34-5p

0.2

125.1

−5.82

BnaA05g29360D,BnaA09g14240D,BnaA05g30840D,BnaA01g24360D,BnaA07g34690D,BnaC03g41430D,BnaA03g35600D

bna-novel_31-5p

4.7

53.3

−2.81

BnaC01g21190D,BnaC03g13660D,BnaA09g19450D,BnaA05g09930D

6251

bna-novel_28-3p

5.2

38.3

−2.48

BnaA09g36810D,BnaA10g16750D

bna-miR408-5p

23.8

60.6

−1.15

BnaA02g05230D,BnaA10g18650D,BnaA08g27620D,BnaAnng21260D,BnaA10g02870D,BnaA08g00080D

bna-miR398a-3p

0

5.1

−2.10

BnaA05g02320D,BnaA06g14440D,BnaA08g08840D

bna-novel_34-5p

23.2

57.5

−1.11

BnaA02g05230D,BnaA10g18650D,BnaC01g01790D,BnaA10g10960D,BnaAnng21260D,BnaA10g14890D,BnaA08g27620D,BnaA07g15260D,BnaAnng36200D,BnaA10g15730D,BnaA02g11840D,BnaA10g25360D,BnaA10g02870D,BnaA08g00080D,BnaA10g17820D,BnaA09g25870D

6284

bna-miR394a/b

195.2

87.5

1.04

BnaA05g16640D,BnaA01g24160D,BnaA05g11890D,BnaC02g17150D,BnaAnng09250D,BnaA08g10740D,BnaA07g36430D,BnaC02g29160D,BnaA06g08380D,BnaC01g07190D,BnaA05g00820D,BnaA05g13120D,BnaC01g39490D,BnaC01g16400D,BnaA02g34270D,BnaC02g43190D,BnaA01g33370D,BnaC01g39860D,BnaA08g27810D

bna-novel_33-3p

30.2

8.4

1.76

BnaA02g26940D,BnaA09g44810D,BnaA06g12040D,BnaA06g14250D,BnaA02g23840D,BnaA10g26020D,BnaA03g12030D,BnaA09g06400D,BnaC01g11370D,BnaA10g03030D,BnaA03g05700D,BnaA10g26690D,BnaA05g01020D,BnaA02g00710D

bna-miR159a

2320.1

1295.7

0.82

BnaAnng27960D,BnaA04g18810D,BnaA03g15690D,BnaA07g18670D,BnaA07g12970D,BnaA03g22590D,BnaA07g25350D,BnaA06g20460D,BnaA01g18450D,BnaC01g19500D,BnaA01g16350D,BnaA09g27090D,BnaA06g13170D,BnaA09g42230D,BnaA09g30160D,BnaAnng05670D,BnaA06g18020D,BnaA08g20300D,BnaA02g05410D,BnaAnng21510D,BnaA09g08360D,BnaAnng14630D,BnaA09g02220D,BnaA04g25320D,BnaA09g10390D,BnaA02g30030D,BnaA09g44380D,BnaC01g19800D,BnaA01g35420D,BnaA08g27930D

bna-novel_9-3p

114.4

68.3

0.75

BnaA03g33680D,BnaA08g01260D,BnaA01g05980D,BnaAnng27960D,BnaA03g22590D,BnaA07g12970D,BnaA04g09220D,BnaA07g12970D,BnaA02g05410D,BnaA09g55500D,BnaAnng13060D,BnaA01g23170D,BnaA09g13960D,BnaA09g47880D,BnaA04g01370D,BnaA08g17490D,BnaC02g08160D,BnaA02g33550D,BnaC02g42310D,BnaA10g14420D,BnaA05g16460D,BnaA03g42760D

bna-miR398a-3p

436.2

972.6

−1.06

BnaA08g19040D,BnaC02g28060D,BnaC01g27860D,BnaA10g26450D,BnaC03g13330D,BnaA03g10640D,BnaC02g12070D,BnaC01g18280D,BnaA01g15390D,BnaC02g42090D,BnaAnng31090D,BnaC01g43200D,BnaA05g30940D,BnaA01g29240D,BnaC01g36670D

bna-novel_3-3p

3.1

23.4

−2.43

BnaA05g24640D,BnaA09g38650D,BnaA06g39700D,BnaA09g39360D,BnaA09g54170D,BnaA06g10130D,BnaA10g14600D,BnaC01g22370D,BnaA10g24950D,BnaA10g23300D,BnaA04g14120D,BnaA09g41640D,BnaC01g09540D

bna-novel_5-3p

1.5

13.5

−1.77

BnaA05g24640D,BnaA06g39700D,BnaC02g14840D,BnaA06g10130D,BnaA09g38650D,BnaC01g22370D,BnaA09g39360D,BnaA10g04390D,BnaA09g54170D,BnaA10g14600D,BnaA01g28170D,BnaA06g28940D,BnaA10g23460D,BnaA06g07690D,BnaA10g24950D,BnaA09g01710D,BnaA10g16750D,BnaA10g23300D,BnaC01g09540D

bna-miR408-5p

41.3

94.4

−1.13

BnaA03g22490D,BnaA10g18290D,BnaA07g26450D,BnaA06g37530D,BnaC02g29240D,BnaA05g29880D,BnaA10g21900D,BnaC01g16210D,BnaA02g09770D,BnaA10g23140D,BnaA02g34310D,BnaC02g43230D,BnaA01g08900D,BnaA06g10230D,BnaA10g15210D,BnaA05g14430D,BnaC02g46010D,BnaA03g04360D,BnaA04g08040D,BnaAnng32010D,BnaA03g30060D

bna-novel_1-3p

0.9

7.5

−1.72

BnaA02g30100D,BnaC02g38520D,BnaA10g21900D,BnaAnng29380D

bna-novel_34-5p

1.0

456.9

−6.87

BnaA02g05300D,BnaA05g27100D,BnaA07g28780D,BnaA06g28070D,BnaA09g14240D,BnaA05g30840D,BnaA08g29690D,BnaC03g48340D,BnaA04g22150D,BnaC01g31340D,BnaA01g24360D,BnaA03g23950D,BnaC03g53270D,BnaA06g01440D,BnaA02g14960D,BnaA09g03120D,BnaA09g31030D,BnaC02g03920D,BnaA07g34690D,BnaA03g35600D,BnaA04g02140D,BnaC02g14950D,BnaA03g10460D,BnaAnng21410D