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Fig. 5 | BMC Plant Biology

Fig. 5

From: The legume-specific transcription factor E1 controls leaf morphology in soybean

Fig. 5

E1 represses TCPs transcription by directly binding to the promoter. a. Constructs used for the transient transfection assay. b. Luciferase activity under the control of TCP14 and TCP29 promoters, from three biological replicates. A Student’s t-test was used to detect statistically significant differences. c. Location of the promoters of TCP14 and TCP29 and the amplicons targeted in ChIP-qPCR assay. d. Results of ChIP-qPCR on TCP amplicons in DN50 and E1-OE lines fused with Flag tags. A monoclonal Flag antibody was used for the ChIP assay

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