Fig. 1

The leaf phenotypic characterization of the E1-overexpression (E1-OE) plants in DN50. a. Immuno-blot of FLAG antibodies in DN50 and E1-OE lines. The gel image had high-contrast and the original gel image included in the Additional file 2. b. The expression of E1 in DN50 and E1-OE plants. The soybean TUB (GmTubulin) gene was used as an internal control to normalize gene expression data. The experiment was performed using three biological replicates, each with three technical replicates. Differences between groups were statistically analyzed using Student’s t-test (**P < 0.01). Bars indicate standard error of the mean. c. Top view of DN50 and E1-OE plants. Red boxes indicates that the close-up image of the leaf of DN50 and E1-OE plants. d. Quantification of leaf size in DN50 and E1-OE plants (n = 10). e. Quantification of leaf weight in DN50 and E1-OE plants (n = 10). All values are presented as mean ± standard error of the mean (s.e.m.) (n = 10 plants). Bars indicate the s.e.m. Significant differences were identified by Student’s t-test (**P < 0.01)