Table 1 List of analysed bitter acid biosynthesis gene, trichome-specific transcription factor and reference gene sequences

Bitter acids biosynthesis

HlBCAT1

Branched-chain amino acid aminotransferase 1

002627F.g2 JQ063073

HlVPS

Phloroisovalerophenone synthase

001329F.g74 FJ554588

HlPT1L

2-Acylphloroglucinol 4-prenyltransferase

KM222441

HlPT2

2-Acyl-4-prenylphloroglucinol 6-prenyltransferase

KM222442

HlHS1

Monooxygenase 2 (humulone synthase 1)

010625F.g1 008956F.g7 KJ398144

HlHS2

Monooxygenase 2 (humulone synthase 2)

008118F.g14 KJ398145

Transcription regulation of bitter acid biosynthesis

HlMYB3

Transcription factor HlMYB3

AM501509

HlMYB7

Transcription factor HlMYB7

007341F.g6 FR873650

HlMYB8

Transcription factor HlMYB8

002031F.g25 HG983335

HlMYB78

Transcription factor MYB78

000063F.g63

HlbHLH2

Transcription factor HlbHLH2 (TT8)

000662F.g4 FR751553

HlWRKY1

WRKY transcription factor 1

000029F.g2 CBY88801

Transcription regulation of trichome development

HlETC1

Enhancer of TRY and CPC 1

GAAW01034579

HlCPC

MYB Transcription factor CPC (CAPRICE)

GAAW01028135

HlMYB61

Transcription factor MYB61

000756F.g12

HlMYB5

Transcription repressor MYB5

001020F.g25

HlMYB106

Transcription factor MYB106 (MIXTA)

000018F.g94

HlGL3

Transcription factor GLABRA 3 (HlbHLH4)

001145F.g21 HG983336

WRKY44

WRKY transcription factor 44

002199F.g12

HlGL2

Homeobox-leucine zipper protein GLABRA 2

002700F.g20

HlZHD6

Zinc-finger homeodomain protein 6

000360F.g51

HlHDG11

Homeobox-leucine zipper protein HDG11

004599F.g3

HlHDG2

Homeobox-leucine zipper protein HDG2

000903F.g16

HlPDF2

Homeobox-leucine zipper protein PROTODERMAL FACTOR 2

001505F.g11

HlHB14

Homeobox-leucine zipper protein ATHB-14

GAAW01071051

HlHB51

Homeobox-leucine zipper protein ATHB-51

005711F.g8

HlZFP4

Zinc finger protein 4

005456F.g11

HlZFP5

Zinc finger protein 5

000280F.g32

HlZFP6

Zinc finger protein 5

001196F.g44

HlZFP8

Zinc finger protein 5

001694F.g13

HlGIS3

Zinc finger protein GIS3

000092F.g31

HlYABBY1

Axial regulator YABBY 1

000464F.g10

HlRAV1

AP2/ERF and B3 domain-containing transcription factor RAV1

001017F.g6

HlABIL2

Abl interactor-like protein-2

000482F.g27

HlABIL3

Abl interactor-like protein-3

001806F.g14

HlCYCB2–4

Cyclin-B2–4

001015F.g12

HlCYCNB1

G2/mitotic-specific cyclin-1

006700F.g8

Reference genes

HlTTG1

Protein TRANSPARENT TESTA GLABRA 1 (HlWD40)

002162F.g15 FN689721

HlMYC2

Transcription factor MYC2

001862F.g5

HlPIF4

Transcription factor PIF4

000802F.g1

HlGAPDH

Glyceraldehyde-3-phosphate dehydrogenase

004935F.g2 004935F.g5

HlRH46

DEAD-box ATP-dependent RNA helicase 46

000004F.g75