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Table 3 Diagnostic, species-specific, single nucleotide polymorphism (SNP) sites in the ITS1 and ITS2 regions (with the species-specific letter code followed by the regions numbered 1–9) for Phalaris species, within each species-specific site, specifying the nucleotide substitution (A/C, A/G, A/T, C/A, C/G, C/T, G/A, G/C, T/A, T/C, T/G) and the relative position each site on the ITS1 and ITS2 multialignments (Supplementary Fig. 4). Two species (P. lemmonii, P. angusta) lack diagnostic SNPs, while two species (P. brachystachys, P. canariensis) are indistinguishable (N/A)

From: Variability in ITS1 and ITS2 sequences of historic herbaria and extant (fresh) Phalaris species (Poaceae)

P. truncata Guss. ex Bertol.

T1

A/C 23

T2

G/C 155

T3

T/G 166

T4

T/A or T/C 245

T5

T/A or T/G 381

    

P. brachystachys Link & P. canariensis L.

B-C1

A/G 52

B-C2

C/T 65

B-C3

A/C 88

B-C4

A/C 162

B-C5

C/A 246

B-C6

T/C 315

B-C7

A/C 339

B-C8

A/G 346

B-C9

A/G 349

P. aquatica L.

A1

T/G 45

A2

A/T 132

A3

A/T or A/C 221

A4

C/T or C/A 237

A5

A/C 352

    

P. coerulescens Desf

C1

DEL 426

C2

T/C 424

C3

A/T 414

      

P. minor Retz.

M1

T/C 202

M2

T/G or T/A 252

M3

T/C 373

      

P. paradoxa L

P1

C/G 403

        

P. californica Hook. & Arn.

CA1

G/A or G/C 246

CA2

G/A 416

       

P. caroliniana Walter

CR1

A/T 213

CR2

T/A 385

CR3

A/G 381

      

P. arundinacea L.

AR1

T/G or T/C 198

AR2

C/T 215

AR3

A/G or A/T 252

AR4

G/A 281

AR5

A/C 351

AR6

T/C 337

   

P. lemmonii Vasey

N/A

        

P. angusta Nees ex Trin.

N/A

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