Table 1 Phalaris species historic herbaria specimen data from leaf sample collections at the Bell Museum Herbarium (MIN; University of Minnesota) and Ada Hayden Herbarium (ISC; Iowa State University), DNA concentration (ng/μl) of extracted samples, their corresponding DNA quality readings from the spectrophotometer (optical densities [OD] at two ratios: OD _260/280 and OD _260/230), amplification success (+/−), herbaria accession codes and the National Center for Biotechnology Information (NCBI) GenBank accession numbers sequences

P. angusta Nees ex Trin..

16 May 1936

15.8

1.94

1.44

+

ISC-V-0020926

MN811167.1

16 April 1960

13.8

2.00

1.50

+

ISC-V-0020921

MN811165.1

3 June 1964

14.5

1.97

1.25

+

ISC-V-0020922

MN811168.1

12 April 1970

29.2

1.98

1.89

+

ISC-V-0020920

MN811166.1

3 April 1982

6.1

2.48

1.27

+

ISC-V-0020919

MN811169.1

P. aquatica L.

8 September 1935

34.6

1.95

1.79

+

ISC-V-0021399

MN811171.1

31 August 1959

9.1

2.05

1.13

+

ISC-V-0020927

MN811170.1

8 July 1962

13.9

1.88

0.81

+

ISC-V-0021398

MN811172.1

P. arundinacea L.

30 June 1913

70.8

2.00

1.82

+

71,166

n/a

28 May 1955

6.9

1.5

0.54

+

532,148

MN811175.1

27 August 1983

40.7

1.93

2.42

+

753,216

MN811176.1

26 June 2001

6.3

2.07

2.59

+

484,712

MN811200.1

P. brachystachys Link

8 April 1959

10.2

2.12

2.43

+

ISC-V-0021036

MN811180.1

8 April 1959

10.5

2.37

1.61

+

ISC-V-0021039

MN811179.1

8 April 1959

63.3

1.91

2.41

+

ISC-V-0021035

MN811178.1

10 August 1962

7.8

2.21

3.90

+

ISC-V-0021037

MN811181.1

P. californica Hook. & Arn.

4 May 1903

16.2

2.03

2.11

+

ISC-V-0021040

n/a

24 May 1907

10.6

1.47

0.66

–

ISC-V-0021044

n/a

18 June 1908

19.9

1.86

1.04

+

ISC-V-0021043

MN811182.1

1958

10.1

2.16

2.52

–

ISC-V-0021041

n/a

ND*

8.3

1.78

1.79

–

ISC-V-0021042

n/a

P. canariensis L.

August 1884

26.1

1.91

2.62

+

71,226

n/a

29 July 1886

41.9

1.87

2.39

–

71,229

n/a

12 July 1938

73.1

1.95

2.12

+

367,474

MN811185.1

July 1969

87.7

1.88

2.09

+

619,107

n/a

P. caroliniana Walter

30 May 1940

17.8

2.01

2.07

+

ISC-V-0021097

MN811188.1

28 May 1964

2.8

2.53

1.74

+

ISC-V-0021081

MN811187.1

6 April 1973

7.8

2.28

2.47

+

ISC-V-0021166

n/a

3 May 1982

25.9

1.97

2.17

+

ISC-V-0021080

MN811186.1

P. coerulescens Desf.

24 November 1958

23.8

2.05

1.61

+

ISC-V-0021199

MN811190.1

15 December 1958

5.0

2.60

1.40

+

ISC-V-0021198

n/a

8 April 1959

22.9

2.06

1.93

+

ISC-V-0021204

n/a

8 April 1959

6.6

2.19

1.45

+

ISC-V-0021203

MN811189.1

P. lemmonii Vasey

1888

6.6

1.61

−4.95

–

ISC-V-0021333

n/a

18 April 1946

26.2

2.02

1.89

+

ISC-V-0021334

n/a

15 June 1955

42.9

1.79

0.79

+

ISC-V-0021329

MN811192.1

7 May 1956

9.0

2.11

0.96

+

ISC-V-0021336

MN811191.1

23 May 1980

8.8

1.80

0.63

+

ISC-V-0021328

n/a

P. minor Retz.

May 1882

12.6

1.92

0.88

–

ISC-V-0021344

n/a

April 1901

36.3

1.81

2.07

–

229,774

n/a

12 April 1918

17.0

1.80

0.72

–

ISC-V-0021338

n/a

24 November 1958

12.7

2.11

1.74

–

ISC-V-0021342

n/a

8 April 1959

15.3

2.12

1.70

+

ISC-V-0021341

MN811193.1

P. paradoxa L.

13 May 1914

19.6

2.03

2.20

+

ISC-V-0021360

n/a

4 June 1915

10.9

1.71

0.61

–

ISC-V-0021363

n/a

2 June 1934

71.8

1.68

0.75

+

ISC-V-0021361

MN811195.1

3 May 1940

3.8

1.90

7.05

–

ISC-V-0021362

n/a

8 April 1959

2.5

7.42

0.27

+

ISC-V-0021415

MN811194.1

P. truncata Guss. ex Bertol.

11 November 1958

19.7

1.88

2.68

+

ISC-V-0021377

MN811198.1

24 November 1958

7.7

2.25

2.03

+

ISC-V-0021384

MN811199.1

15 December 1958

30.5

2.06

1.81

+

ISC-V-0021395

MN811197.1

24 December 1958

2.9

2.66

0.38

+

ISC-V-0021373

MN811196.1