Fig. 3

a Examples of PCR amplification results of the plant specific ITS specific region [43] on genomic DNA from panel a. fresh Phalaris canarensis (1; 53.6 ng; PI 578800), P. aquatica (2; 60.7 ng; PI 476287) and P. arundinacea (3; 28.5 ng; PI 422030); b herbarium specimens with a uniform quantity of 50 ng P. canarensis (4; 71,226), P. californica (5; ISC-V-0021043), P. californica (6; ISC-V-0021040), P. caroliniana (7; ISC-V-0021097), P. paradoxa (8; ISC-V-0021360), P. coerulescens (9; ISC-V-0021199), P. minor (10; ISC-V-0021338); c PCR re-amplification result of purified PCR reaction of P. canarensis, 1/50 of previous PCR reaction (B4) as a PCR template. M – DNA ladder, CT – Control. Full-length blots/gels are presented in Supplementary Fig. 2