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Fig. 8 | BMC Plant Biology

Fig. 8

From: Genome-wide identification of the tobacco GDSL family and apical meristem-specific expression conferred by the GDSL promoter

Fig. 8

Construction of recombinant vectors. a: Schematic map of the PNtGDSL127::GUS recombinant vectors. The promoter fragments were inserted at the HindIII and BamHI sites of PBI121. The GUS gene was driven by the PNtGDSL127 promoter and the 5′ deletion promoters seperately. b: Agarose gel separation of PCR product of PNtGDSL127, PNtGDSL127-A, PNtGDSL127-B and PNtGDSL127-C, c: Enzymatic digestion of the recombinant plasmid PNtGDSL127 and PNtGDSL127-C using HindIII and BamHI. d: Enzymatic digestion of the recombinant plasmid PNtGDSL127-A and PNtGDSL127-B using HindIII and BamHI. 22(PNtGDSL127::GUS), 25(PNtGDSL127-A::GUS), 27(PNtGDSL127-B::GUS), and 30(PNtGDSL127-C::GUS) independent transformants of each construct were obtained seperately

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BMC Plant Biology

ISSN: 1471-2229

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