Fig. 3

Insertional differences between FTL1 amplicons A1 and A2. Illustration of differences between the A1 and A2 amplicons. In the figure, F and R bracket the amplification region of the FTL1 gene from forward to reverse FTL primers, black segments indicate sequence similarity between the amplicons, yellow segments in the A1 amplicon indicate insertion and the yellow arrows above the A2 amplicon indicate the insertion position in the A2 amplicon relative to the A1 amplicon. The black segments also include small indels that contribute to minor differences in length of indicated segments between the two amplicons. The short, unique sequences found uniquely in the A2 amplicon are not illustrated in this figure