Fig. 5

The axillary bud growth vigor, CTK contents in the three tissues, and the expression of the total CsA-IPT5 transcripts and CsA-IPT5 protein in the axillary bud of the first node below the cuttings induced by the second pruning. A The growth vigor of the axillary bud at 16 d after pruning. B-E Contents of tZ- and iP-type CTKs in the different tissues at 8 d after pruning. Data are means ±SD (n = 3). For each figure, letters indicate significant differences in the CTK concentration between control and pruning in each tissue (P < 0.05, Duncan’s multiple range test). F Expression of the total CsA-IPT5 transcripts in the axillary bud at different time points after pruning. G, H Western blot (G) and ‘CsA-IPT5/Actin’ (H) which showed the CsA-IPT5 protein expression levels in the axillary bud at different time points after pruning. Data are means ±SD (n = 3). Letters indicate significant differences of CsA-IPT5/Actin between control and pruning at each time point (P < 0.05, Duncan’s multiple range test)