Fig. 4

Functional characterization of the ramie KNOX protein whole_GLEAN_10029667. a Phylogenetic relationships among Arabidopsis KNOX proteins and whole_GLEAN_10029667. b Microscopic examination of transverse sections of the stems of whole_GLEAN_10029667-overexpressing (left) and wild-type Arabidopsis (right) plants, at ×100 magnification. The red arrows indicate fiber cells. The scale bar represents 200 μm. c Verification of interactions with whole_GLEAN_10029667 from a one-to-one Y2H assay. The left and right rows represent the yeast colonies cultivated on SD/-Leu/-Trp/X-α-Gal and SD/-Leu/-Trp/-His/-Ade/-Aba/X-α-Gal agar plates, repressively. The colonies in the first line are yeasts containing both pGBKT7-whole_GLEAN_10029667 (pGBKT7/667) and pGADT7- whole_GLEAN_10002382 (pGADT7/382) constructs, whereas colonies in the second line are those of control yeast transformed with an empty pGBKT7 vector. The colonies in the third line indicate the interaction of whole_GLEAN_10029667 and whole_GLEAN_10021119 by transferring both pGBKT7-whole_GLEAN_10029667 (pGBKT7/667) and pGADT7- whole_GLEAN_10021119 (pGADT7/119) constructs into yeast, whereas the colonies in the fourth line are those of the control into which an empty pGBKT7 vector was transferred. The fifth and sixth lines show the positive and negative controls used in the Y2H assay, respectively