Fig. 3

Accumulation of salicylic acid (SA) and the expressions of genes related to SA accumulation in tomato plants after infection with Cmm. a The expression of genes responsible for SA accumulation in tomato cotyledon after infection with Cmm. The transcript levels were quantified by quantitative reverse transcription-polymerase chain reaction and were expressed relative to the transcript level at 0 day, which was assumed to be one. Data are represented as mean values ± standard deviation for three replicates. b The level of SA accumulation in the tomato cotyledons after infection with Cmm. Data are represented as mean values ± standard deviation for six replicates. c Changes in the expression of genes involved in the possible SA biosynthetic pathway. Genes marked with an asterisk were upregulated in the tomato cotyledon after infection with Cmm. The upregulated genes identified by RNA-sequencing analysis (Table S3) were four phenylalanine ammonia lyase (PAL) genes (SlPAL2/Solyc09g007900, SlPAL4/Solyc09g007920, SlPAL5/Solyc09g007910, and SlPAL6/Solyc05g056170), three 4-coumarate:CoA ligase homologs (Sl4CL/Solyc03g117870, Solyc06g068650, and Solyc12g042460), and three 3-hydroxyacyl-CoA dehydrogenase homologs (Solyc07g019670, Solyc12g007170, and Solyc08g068390)