Fig. 2

Phylogenetic tree, structural gene features and conserved protein motifs of the Gγ subunit genes in the eight Rosaceae species. A The phylogenetic tree was generated using the amino acid sequences encoded by each of 35 Gγ subunit genes with the Neighbor-Joining method and 1,000 bootstrap replicates. Different colors in the branches indicated the different subunit type. B Gene structural analysis, the orange boxes, black lines and green boxes in the gene structural diagram represent coding sequences (CDS), introns and untranslated regions (UTRs), respectively; gene models are drawn to scale as indicated on the x-axis. C Conserved motif analysis: fifteen distinct motifs (motifs 1 to 15) were identified with the MEME tool and the representation of each motif was illustrated with a different color. The lengths and positions of the colored blocks correspond to the lengths and positions of the motifs in the individual protein sequences, respectively