Fig. 1

OsANN4 responds to exogenous abscisic acid in rice. a Schematic diagram of the gene structure and protein domain of OsANN4. Exons, introns and protein domains are indicated by black boxes and dotted lines between black boxes and orange boxes, respectively. b Phylogenic tree of OsANN4 and the Arabidopsis ortholog AtANNs. The phylogenetic trees were constructed with Mega X using the maximum likelihood method. OsANN4 is marked with a red dot. c OsANN4 transcript expression levels under ABA treatment. OsACTIN1 was used as the internal control. d Immunoprecipitation of OsANN4 in OsANN4-overexpressing lines treated with ABA for 1 h, 3 h and 6 h. Values represent the means ± SD from three independent repeats, and different letters indicate significant differences (one-way ANOVA, P<0.05)