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Fig. 5 | BMC Plant Biology

Fig. 5

From: The steroid-inducible pOp6/LhGR gene expression system is fast, sensitive and does not cause plant growth defects in rice (Oryza sativa)

Fig. 5

Expression characteristics of the pOp6/LhGR system induced in soil-grown plants by painting and watering. Four-week-old rice plants grown in soil were induced either with TA or Dex by (A) painting with 10 μM solution supplemented with 0.1% Tween-20 or (B) watering with 30 μM solutions. The treatment was repeated twice at 0 h and 24 h later. Leaves were sampled and GUS activity determined 24 h and 72 h following first application of the inducer. NT and pZmUbi::GUS plants were used as controls. Three plants from segregating populations were used for each treatment. The error bars represent SD. C-K Four-week-old plants (line 121C) were induced as above except the treatment was repeated 48 h after first induction. Leaves were imaged using a confocal laser scanning microscope at 24 h and 96 h post-induction, YFP signal in green, chlorophyll autofluorescence in red. Scale bar is 20 μm

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