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Fig. 2 | BMC Plant Biology

Fig. 2

From: The steroid-inducible pOp6/LhGR gene expression system is fast, sensitive and does not cause plant growth defects in rice (Oryza sativa)

Fig. 2

Reliability of inducible expression in subsequent generations and GUS activity in rice. A Ten-day-old rice seedlings (line 65) of generation T1, T2 (A and B) and T3 (A1, A2 and B1, B2) histochemically stained for GUS activity induced by germinating and growing the seedlings on ½ MS plates containing 30 μM Dex or control DMSO (−Dex) for 10 days. Seedlings of pZmUbi::GUS were included as a control for the GUS staining. Scale bar represents 1 cm. B, C GUS activity determined fluorometrically in roots (B) and shoots (C) of 7-day-old rice seedlings (lines 65 and 121, T1 and T2) germinated and grown on ½ MS plates containing 30 μM Dex or control DMSO (−Dex) for 7 days. Seedlings of NT and pZmUbi::GUS were included as controls. The error bars represent SD

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