Fig. 6

GhWRKY1-like positively regulated the expression of ABA biosynthesis related genes AtNCED2, AtNCED5, AtNCED6 and AtNCED9. A Yeast one-hybrid assay showing the binding of GhWRKY1-like to the promoters of AtNCED2, AtNCED5, AtNCED6 and AtNCED9. Empty pHisi-1 vector was used as a negative control. Yeast cells were grown on SD-Leu-His containing different concentration different concentrations of 3-amino-1,2,4-triazole (3-AT) for 3 to 5 d at 30 °C. B Diagram of W-box positions in the promoters of AtNCED2, AtNCED5, AtNCED6 and AtNCED9. C Diagram of the effector and reporter constructs for the transactivation assay. The reporter carries a renilla luciferase gene under the control of the 35S promoter and a firefly luciferase reporter gene under the control of the promoter of AtNCED2, AtNCED5, AtNCED6 or AtNCED9. 35S, promoter of cauliflower mosaic virus 35S RNA gene; REN, Renilla luciferase; CaMV term, cauliflower mosaic virus terminator; LUC, firefly luciferase. D Translational changes of the reporters in the presence of GhWRKY1-like in Arabidopsis protoplasts. The LUC/REN activity ratios are the means ± SD; n = 3. Comparisons were performed with Student’s t test. *, P < 0.05; **, P < 0.01