Table 1 Primer sequences used in this study
From: Overexpression of a carrot BCH gene, DcBCH1, improves tolerance to drought in Arabidopsis thaliana
Gene | Function | Forward primer (5′-3′) | Reverse primer (5′-3′) | Reference |
|---|---|---|---|---|
DcBCH1 | Full lengths clone | ATGGCGGCCGGAATTTCGGCG | CTATGATCGGCTAGCTCTGGA | – |
DcBCH1 | Overexpression vector conduction | TTTACAATTACCATGGGATCCATGGCGGC CGGAATTTCGGCG | ACCGATGATACGAACGAGCTCCTATGATCGGC TAGCTCTGGA | – |
AtGGPS | RT-qPCR | TGCTTGTGAACTCGTCGGAGGT | CACGGCGGAGATCGTCGTTATC | – |
AtPSY | RT-qPCR | TGCTGCTCTCGCTGATACAGTTG | CCTCTTCTCGCATCTTCGCCTAC | Yin et al. 2020 [60] |
AtPDS | RT-qPCR | GCTCAATGACGATGGCACGGTTA | CGGCATACACGCTCAGAAGGTTAC | Yin et al. 2020 [60] |
AtZDS | RT-qPCR | GACATTCGCAACCTTGACAGCATAA | CCTTGAGCATACGCAACAGAGAAG | Yin et al. 2020 [60] |
AtZISO | RT-qPCR | CTAACCTCACCTTAATCCGCCGTAT | AAGAGAACAACTCCAAGGACAACAC | Yin et al. 2020 [60] |
AtCRTISO | RT-qPCR | TCCTCAGCATTCCAACCATTCTTGA | GCGAGTGTCCTTAGCCAACCAA | Yin et al. 2020 [60] |
AtLCYE | RT-qPCR | GTAGTGTCAGAGCTAGCGGC | AAGGCTAAACCAGCAGGACC | – |
AtLCYB | RT-qPCR | AGATGGAATGTGCCTTGTTGTTGGA | ACTCGGAGATGTTGATTGCGGTTC | Yin et al. 2020 [60] |
AtLut1 | RT-qPCR | GCGTCTCTATCCTCATCCTCCT | GTATGGTTGCTCCTGTGGTCAT | – |
AtCCD1 | RT-qPCR | TGGCAGCAGCATCATCTCAGTC | ATCACGGATGGGAGCGAAGTTG | – |
AtCCD4 | RT-qPCR | CGGCACTCTTCCACTGTCACTT | TTCGGTTAATCGGACGGCGTAG | – |
AtZEP | RT-qPCR | TCTTCGTTGACATTGCTATGCCATC | CGCCGCCTTCTTATCTGAACCA | Yin et al. 2020 [60] |
AtNCED3 | RT-qPCR | GGAGATGGCTTGGTGGCAATCA | GCTTCTCGTGGCTGACAAGGAA | – |