Table 1 Gene-specific primers used for cloning and qRT-PCR
Gene | Forward primer (from 5′ to 3′) | Reverse primer (from 5′ to 3′) |
|---|---|---|
Ckγ-ECS(cloning) | ATGGCTATCATCTCCCGA | ATAAAGCAACCTCAAATA |
Ckγ-ECS | TGCGAGCTCATGGCTATCATCTCCCGA | CGGCTGCAGATAAAGCAATTCCTCAAATA |
qCkUB | GACTTTGACCGGGAAGACCA | CACCACGAAGACGGAGCACA |
qCkFAMA | TGAACGTAGGAACCAGGCA | AACAAGAGGAAGAGACCAAGAAG |
qCkAXR3 | CTGGAAGATGTTTGTGGAATCA | AGGGACATTTACATCGTCATTACAC |
qCkARF5 | GTCAGTGTGCCTCGTCGTCTC | TGTCCTCCCCGTTAATCTTCA |
qCkSTOMAGEN | AATCCTCACAACCTCAAAGAGAACT | TGGGGTCATTTCCCTCCACAGG |
qCkγ-ECS | AAGTTGGTTCTCTCGGGCT | CGGTGCTTATCAGTATCGGTC |
qAtActin7 | CACTACCGCAGAACGGGAAA | GCGATGGCTGGAACAGAACC |
qAtFAMA | CAGCAACATCAACTCTCTCCTTCC | TCTGGTCTGGTTCAACGCAA |
qAtAXR3 | CTCTTTTACCATGGGCAAACATGGA | AGGGAACATAGTCCCAGCTATTCA |
qAtARF5 | TAATGGGGAATGGAGGTTTG | GTGTAGAGTTCTCAGGTTTAGCAG |
qAtSTOMAGEN | TAGGGTCGACAGCACCAACTTGTAC | TCATTTCCTTCGACTGGAACTTGCT |
qAtERF1 | TTCTTCTCCTTGCCTTTTTCC | AACCTAGACCCAGCCACCTG |
qAtγ-ECS | CTGTTAAGAGGAGTAAGAGAGGGC | CCAGAGGCAAGATAGGCAATG |