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Fig. 5 | BMC Plant Biology

Fig. 5

From: PbLAC4-like, activated by PbMYB26, related to the degradation of anthocyanin during color fading in pear

Fig. 5

Anthocyanin degradation activity assay. a The activity assay of the crude enzyme to degrade anthocyanin in leaves of various varieties was carried out with anthocyanin as a substrate. b The SDS-PAGE gel of prokaryotic expression PbLAC4-like protein (M stood for the marker, Eui was pET28a (+) empty vector without induction, Ei was induced pET28a (+) empty vector, Eip and Eis were precipitation and supernatant of induced pET28a empty vector respectively, Lui stood for pET28a (+) -PbLAC4-like vector without induction, Li was induced pET28a (+) -PbLAC4-like vector and Lip and Lis was precipitation and supernatant of induced pET28a (+) -PbLAC4-like vector respectively). c The enzyme activity test of prokaryotic expressed PbLAC4-like protein was carried out with anthocyanin as a substrate. The significant difference was determined by t test for three replicates: *P < 0.05; **P < 0.01. Error bars represent the means ± SEM of three biological replicates

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