Fig. 4

Transient transformation assays in the ‘Palacer’ fruitlet peel to verify the function of PbLAC4-like. a The phenotype of overexpressing PbLAC4-like (OE-PbLAC4-like) in ‘Palacer’ fruit, taking empty vector containing GUS (Empty) as the control. b Anthocyanin content and the expression level of PbLAC4-like gene in OE-PbLAC4-like and Empty. The significant difference was determined by t test for three replicates: *P < 0.05; **P < 0.01. Error bars represent the means ± SEM of three biological replicates