Fig. 4
From: Molecular mechanism of modulating miR482b level in tomato with botrytis cinerea infection
Dicer splicing activities of two pri-miR482b isoforms. (A) Splicing efficiencies of two pri-miR482b isoforms in vitro. Land 1: pri-miR482b-x1; Lane 2: pri-miR482b-x2; Lane 3: pri-miR482b-x1 with 2 mg/mL protein extraction; Lane 4: pri-miR482b-x2 with 2 mg/mL protein extraction. (B) Expression levels of pri-miR482b-x1 in pri-miR482b-x1 OE plants and the expression level of pri-miR482b-x2 in pri-miR482b-x2 OE plants via semi-quantification RT-PCR. Lanes 1–3: three OE lines of pri-miR482b-x1; Lanes 4–6: three OE lines of pri-miR482b-x2. C-D) Expression levels of pre-miR482b (C) and miR482b (D) in pri-miR482b-x1 and pri-miR482b-x2 OE plants via quantification RT-PCR. AtUBQ10 was used as the internal control. Results are expressed as means ± SD of three biological replicates. Asterisks indicates a significant difference (P < 0.05) compared with the corresponding OE_pri-miR482b-x1-1