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Fig. 2 | BMC Plant Biology

Fig. 2

From: A rice mTERF protein V14 sustains photosynthesis establishment and temperature acclimation in early seedling leaves

Fig. 2

V14 affects the intercistronic cleavage of the rpoB-rpoC1-rpoC2 precursor, psbD transcript abundance, and PsbA and PsaB accumulations during early seedling development. A Expression analysis of the chloroplastic genes by qRT-PCR in seedlings (L1) grown at 25 °C. The stably expressed chloroplastic gene psbE was used for normalization. Only the genes showing significant changes in v14 and some of the “unchanged” genes are shown here. The significance compared to T65 was analyzed by t test (n = 3). *, P < 0.05; **, P < 0.01. B Immunoblotting analysis of chloroplastic proteins in L1 and L3 with commercially available antibodies. Arrows indicate reduced or no accumulation of the proteins. A stably-expressed plastid protein NdhF was used as an internal control. C Aberrant accumulation of the precursor transcripts covering the two intercistronic regions (P1 and P2) of rpoB-rpoC1-rpoC2 in L1 (grown at 25 °C) of v14, as indicated by semi-quantitative RT-PCR (31 cycles). The cleavage assays for the two intercistronic regions (P3 and P4) of psaA-psaB-rps14 were used as the control. The positions of the primers are designated relative to the start codon of the ORFs downstream or those where they are located. D Significantly higher accumulation of the unprocessed precursor rpoB-rpoC1-rpoC2 in L1 of v14 as compared to T65, as shown by Northern hybridization. L1 and L3, the first and the third true leaf, respectively. (B), (C), and (D), The images presented here are the representatives of three biological repeats

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