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Table 1 Fatty-acid composition of various lipid headgroup classes under each temperature treatment

From: Alterations in the leaf lipidome of Brassica carinata under high-temperature stress

Lipid Headgroup Class

Acyl Chain

Amount/extracted dry mass (normalized mass spectral signal mg-1)

Optimal Temperature (23/15°C)a

High Temperature-1 (33/25°C)

High Temperature-2 (38/30°C)

DGDG

16:0

1.787±0.2152c

3.882±0.2152b

4.567±0.2152a

16:3

0.4251±0.0404a

0.3327±0.0404ab

0.2674±0.0404b

18:1

0.0203±0.0089c

0.0935±0.0089b

0.1383±0.0089a

18:2

0.8069±0.0653a

0.1819±0.0653b

0.2997±0.0653b

18:3

20.06±1.599a

15.82±1.599a

18.07±1.599a

MGDG

16:0

0.3512±0.1176c

1.299±0.1176b

2.008±0.1176a

16:3

22.88±1.924a

25.11±1.924a

23.25±1.924a

18:2

1.226±0.1269a

0.6864±0.1269b

1.133±0.1269a

18:3

41.78±3.507a

48.98±3.507a

51.58±3.507a

PG

16:0

0.3323±0.1488b

1.421±0.1488a

1.554±0.1488a

16:1

1.160±0.1371b

1.357±0.1371ab

1.586±0.1371a

18:0

0.0139±0.0114b

0.0882±0.0114a

0.0578±0.0114a

18:1

0.0954±0.0614b

0.5550±0.0614a

0.5750±0.0614a

18:2

0.0043±0.0017b

0.0137±0.0017a

0.0104±0.00173a

18:3

1.081±0.1208a

1.165±0.1208a

1.153±0.1208a

PC

16:0

2.974±0.4223b

4.285±0.4223a

3.927±0.4223ab

16:1

0.0278±0.0046b

0.0440±0.0046a

0.0349±0.0046ab

18:0

0.0129±0.0076b

0.0627±0.0076a

0.0718±0.00756a

18:1

0.0321±0.0540b

0.3673±0.0540a

0.4209±0.0540a

18:2

1.222±0.3178b

3.664±0.3178a

3.090±0.3178a

18:3

7.404±0.6542a

6.490±0.6542ab

4.779±0.6542b

20:3

0.0156±0.0020a

0.0155±0.0020a

0.0098±0.0020a

PE

16:0

2.386±0.3928a

2.452±0.3928a

2.446±0.3928a

16:1

0.0146±0.0025a

0.0164±0.0025a

0.0155±0.0025a

18:1

0.0106±0.0047b

0.0379±0.0047a

0.0492±0.0047a

18:2

1.168±0.2548b

2.222±0.2548a

2.070±0.2548a

18:3

3.921±0.5817a

3.328±0.5817a

2.607±0.5817a

20:3

0.0121±0.0015a

0.0108±0.0015ab

0.0065±0.0015b

22:0

0.0059±0.0014b

0.0141±0.0014a

0.0122±0.0014a

24:0

0.0187±0.0020a

0.0135±0.0020a

0.0144±0.0020a

PA

16:0

0.5393±0.2988b

2.253±0.2988a

2.499±0.2988a

16:1

0.0083±0.0051b

0.0354±0.0051a

0.0399±0.0051a

18:1

0.0016±0.0302b

0.1435±0.0302a

0.2240±0.0302a

18:2

0.1778±0.1971b

1.576±0.1971a

1.723±0.1971a

18:3

1.064±0.3434b

2.765±0.3434a

2.598±0.3434a

PI

16:0

1.570±0.2148b

2.704±0.2148a

3.031±0.2148a

16:1

0.0088±0.0018b

0.0105±0.0018ab

0.0130±0.0018a

18:0

0.0050±0.0040b

0.0441±0.0040a

0.0532±0.0040a

18:1

0.0037±0.0047b

0.0313±0.0047a

0.0430±0.0047a

18:2

0.2405±0.0793b

1.147±0.0793a

1.375±0.0793a

18:3

1.442±0.1587a

1.701±0.1587a

1.803±0.1587a

  1. Lipid molecular species for which the acyl-chain composition is unambiguous were used in this analysis. For DGDG, this included 34:6 (16:3/18:3), 34:3 (16:0/18:3), 34:1 (16:0/18:1), 36:6 (18:3/18:3), and 36:5 (18:3/18:2). For MGDG, 34:6 (16:3/18:3), 34:3 (16:0/18:3), 36:6 (18:3/18:3), and 36:5 (18:3/18:2). For PG, 32:1 (16:0/16:1), 32:0 (16:0/16:0), 34:4 (16:1/18:3), 34:1 (16:0/18:1), 34:0 (16:0/18:0), 36:6 (18:3/18:3), and 36:5 (18:3/18:2). For PC, 32:0 (16:0/16:0), 34:4 (16:1/18:3), 34:3 (16:0/18:3), 34:2 (16:0/18:2), 34:1 (16:0/18:1), 36:6 (18:3/18:3), 36:5 (18:3/18:2), 36:1 (18:0/18:1), and 38:6 (18:3/20:3). For PE, 34:4 (16:1/18:3), 34:3 (16:0/18:3), 34:2 (16:0/18:2), 34:1 (16:0/18:1), 36:6 (18:3/18:3), 36:5 (18:3/18:2), 38:6 (18:3/20:3), 40:2 (18:2/22:0), and 42:2 (18:2/24:0). For PA: 32:0 (16:0/16:0), 34:4 (16:1/18:3), 34:3 (16:0/18:3), 34:2 (16:0/18:2), 34:1 (16:0/18:1), 36:6 (18:3/18:3), and 36:5 (18:3/18:2). For PI: 34:4 (16:1/18:3), 34:3 (16:0/18:3), 34:2 (16:0/18:2), 34:1 (16:0/18:1), 36:6 (18:3/18:3), 36:5 (18:3/18:2), and 36:2 (18:2/18:0). Acyl-chain amounts are shown as least-squares means with standard error. For each acyl chain, least-squares means with different letters are significantly different according to Fisher’s least significant difference test at α = 0.05
  2. DGDG digalactosyldiacylglycerol, MGDG monogalactosyldiacylglycerol, PA phosphatidic acid, PC phosphatidylcholine, PE phosphatidylethanolamine, PG phosphatidylglycerol, PI phosphatidylinositol
  3. aday/night temperatures