Fig. 3

Gene expression of miR399-mediated Pi signaling related genes in response to Pi deficiency. a-b TaPH1; c-d TaIPS1; e-f TaSPX3; g-h TaPHO2 in the shoots and roots of CS and KN199 after 7 days’ treatment on Pi-sufficient (+Pi, white bars) or Pi-deficient (−Pi, black bars) solutions. The gene expression levels were normalized to the internal control of TaAPT1. The experiments were done using three biological replicates for transcript analysis with three technical replicates for each cDNA sample. Data represent mean values ± SD (n = 3). Columns with different letters indicate significant differences using the least significant difference multiple range test (p < 0.05)