Fig. 7

E3 ubiquitin ligase activity of VyRCHC114. a Determination of E3 ubiquitin ligase activity of VyRCHC114; an immunoblot analysis was performed with the ubiquitin antibody (right) and MBP antibody (left). b Determination of E3 ubiquitin ligase activity of VyRCHC114 mutants; an immunoblot analysis was performed with ubiquitin antibody (right) and MBP antibody (left). c Schematic diagram of VyRCHC114 C3H2C3 domain and putative mutation sites. C328S and N355A affect a non-conserved site of the VyRCHC114 C3H2C3 domain. Mutations in C320S and H341A affect the ubiquitin activity of VyRCHC114