Fig. 2
From: RSAtrace3D: robust vectorization software for measuring monocot root system architecture

Schematic diagram of the process and data flow in RSAtrace3D. In the segmentation process, CT images are processed using root segmentation software to make root-segmented images. In vectorization process, the root images are imported as a 3D volume image. Besides, operators indicate where the roots are by registering the nodes, which are interpolated and smoothly connected to make a vector. The vectorized data are exportable as rinfo files (JSON format). In the calculation process, root- and RSA-related traits are calculated using the vectorized data