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Table 5 Double bond index (DBI) of each main membrane lipid class in C. bifida and C. panzhihuaensis treated at low temperatures

From: Differences in lipid homeostasis and membrane lipid unsaturation confer differential tolerance to low temperatures in two Cycas species

Lipid class Species Treatment    
   Control Cold Freezing Recovery
   DBI    
PA C. bifida 1.57 ± 0.40a 1.48 ± 0.09a 1.53 ± 0.05a 1.60 ± 0.23a
  C. panzhihuaensis 1.87 ± 0.12b 2.02 ± 0.13a* 1.81 ± 0.07b* 1.95 ± 0.11ab*
PC C. bifida 2.03 ± 0.30a 2.26 ± 0.32a 2.19 ± 0.21a 2.70 ± 0.35a
  C. panzhihuaensis 2.84 ± 0.37b* 3.69 ± 0.14a* 2.43 ± 0.16b 2.55 ± 0.49b
PE C. bifida 2.43 ± 0.38a 2.40 ± 0.22a 2.34 ± 0.08a 2.57 ± 0.09a
  C. panzhihuaensis 3.08 ± 0.23b* 3.53 ± 0.22a* 2.92 ± 0.10b* 2.98 ± 0.29b*
PG C. bifida 1.74 ± 0.23a 1.75 ± 0.19a 1.82 ± 0.10a 1.71 ± 0.06a
  C. panzhihuaensis 1.69 ± 0.18c 1.97 ± 0.09ab 2.02 ± 0.03a* 1.85 ± 0.06b*
PI C. bifida 1.65 ± 0.33a 1.51 ± 0.08a 1.59 ± 0.06a 1.48 ± 0.06a
  C. panzhihuaensis 1.94 ± 0.08b 2.05 ± 0.08a* 2.09 ± 0.06a* 2.06 ± 0.04a*
PS C. bifida 0.10 ± 0.02b 0.37 ± 0.13a 0.36 ± 0.16a 0.29 ± 0.04ab
  C. panzhihuaensis 0.39 ± 0.25b 0.80 ± 0.18a* 0.10 ± 0.04c* 0.25 ± 0.20bc
Phospholipids C. bifida 1.70 ± 0.35a 1.67 ± 0.12a 1.72 ± 0.10a 1.80 ± 0.06a
  C. panzhihuaensis 2.10 ± 0.19b 2.50 ± 0.14a* 2.06 ± 0.05b* 2.13 ± 0.20b*
MGMG C. bifida 1.58 ± 0.49a 1.92 ± 0.67a 2.03 ± 0.39a 1.44 ± 0.13a
  C. panzhihuaensis 1.93 ± 0.18b 2.44 ± 0.22a 2.02 ± 0.23b 2.29 ± 0.40ab*
MGDG C. bifida 4.53 ± 1.07a 4.70 ± 0.41a 4.80 ± 0.26a 4.08 ± 0.14a
  C. panzhihuaensis 5.27 ± 0.13c 5.46 ± 0.08b* 5.77 ± 0.08a* 5.51 ± 0.13b*
DGDG C. bifida 2.93 ± 0.44b 2.82 ± 0.26b 3.11 ± 0.33b 3.69 ± 0.12a
  C. panzhihuaensis 3.35 ± 0.18c 3.88 ± 0.18a* 3.49 ± 0.06bc* 3.56 ± 0.13b
SQDG C. bifida 6.96 ± 0.03a 6.95 ± 0.03a 6.95 ± 0.01a 6.97 ± 0.00a
  C. panzhihuaensis 6.89 ± 0.09a 6.95 ± 0.03a 6.94 ± 0.01a 6.95 ± 0.01a*
Saccharolipids C. bifida 5.56 ± 0.34ab 5.32 ± 0.24b 5.22 ± 0.06b 5.75 ± 0.09a
  C. panzhihuaensis 5.55 ± 0.12a 5.51 ± 0.02a 5.84 ± 0.17a* 5.70 ± 0.36a
Total C. bifida 3.99 ± 0.46ab 3.55 ± 0.16b 3.69 ± 0.30b 4.39 ± 0.17a
  C. panzhihuaensis 4.55 ± 0.07b 4.79 ± 0.09a* 4.59 ± 0.06b* 4.72 ± 0.20ab*
  1. DBI = Σ(N× mol% lipid)/100, where N is the number of double bonds in each lipid molecule. Different letters in the same row indicate significant differences between treatments within species and * indicates significant difference between species within treatment (P < 0.05). Data are mean ± standard deviation (n = 5)
  2. DGDG Digalactosyldiacylglycerol, MGDG Monogalactosyldiacylglycerol, MGMG monogalactosylmonoacylglycerol, PA Phosphatidic acid, PC Phosphatidylcholine, PE Phosphatidylethanolamine, PG Phosphatidylglycerol, PI Phosphatidylinositol, PS Phosphatidylserine, SQDG Sulphoquinovosyldiacylglycerol