Fig. 4
From: Enhancement of plant cold tolerance by soybean RCC1 family gene GmTCF1a

The relative expression level of GmTCF1a in soybean as determined by qRT-PCR. A-D Relative expression of GmTCF1a in response to cold (4 °C), ABA (100 μM ABA), PEG (15% PEG8000) and NaCl (200 mM NaCl) treatment at 0, 1, 3, 6, 12, and 24 h, respectively. E Expression of GmTCF1a in leaves, stems, roots and nodules with (Cold) or without (Normal) cold treatment at 4 °C for 12 h. The soybean 18S rRNA gene was used as a reference gene. These experiments were repeated three times and error bars represent the standard deviation between three biological replicates