Fig. 8
From: Regulation of wound ethylene biosynthesis by NAC transcription factors in kiwifruit
Electrophoretic mobility shift assays (EMSA) of AcACS1 promoter fragments (27 bp) with recombinant NAC1–4 proteins. The DNA binding domains of NAC1–4 (described in Nieuwenhuizen et al. 2015 [52]) were over-expressed in Escherichia coli as Maltose Binding Protein (MBP)-tagged fusion proteins and purified by amylose resin affinity purification and EMSA was run according to Nieuwenhuizen et al. (2015) [52]. Wt = wildtype double-stranded DNA probe with putative NAC palindromic binding site (underlined): CATTATACGTATAGTCAACCACATAAC. Mut = mutated double-stranded DNA probe with randomly mutated NAC binding site (italic/underlined): CATCGATCCATCTGTCAACCACATAAC. NAC = MBP-NAC1, −NAC2, −NAC3 or -NAC4