Fig. 5From: A single amino acid residue substitution in BraA04g017190.3C, a histone methyltransferase, results in premature bolting in Chinese cabbage (Brassica rapa L. ssp. Pekinensis)Subcellular localization analysis of BrEBM3. The 35 S:GFP-BrEBM3, 35 S:GFP-Brebm3 and blank 35 S:GFP vectors were transiently expressed in Arabidopsis mesophyll cell protoplasts. The nuclear localization signal (NLS) protein fused with mKate was used as a nucleus marker. Confocal images were captured 24-48 h after inoculation and observed under a confocal laser-scanning microscope. The merged images include GFP channel (green), mKate channel (red), chloroplast autofluorescence channel (pink) and bright field. Scale bar, 10 μmBack to article page