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Fig. 5 | BMC Plant Biology

Fig. 5

From: A cysteine-rich receptor-like protein kinase CaCKR5 modulates immune response against Ralstonia solanacearum infection in pepper

Fig. 5

CaHDZ27 binds to the CAATTATTG motif in the promoter of CaCKR5. a EMSA was used to analyze the interaction of CaHDZ27-GST and a Cy5-labeled probe in vitro. The probe sequences containing the CAATTATTG motif in the CaCKR5 promoter are shown. CaHDZ27-GST purified protein was incubated with Cy5-fluorochrome labeled wild-type probe. Non labeled probes at 10- and 100- fold concentrations were added for the competition test. b Schematic representation of the CaCKR5 promoter used for ChIP-qPCR analysis. The black line indicates the sequence region that was used for ChIP-qPCR. c, ChIP-qPCR assay was performed to verify the binding between CaHDZ27 and CaCKR5 promoter in vivo. DNA samples were co-immunoprecipitated with anti-HA antibody. The ChIP value was normalized to its respective input DNA value. Different letters indicate significant differences, as determined by the LSD test (P < 0.01)

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