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Fig. 3 | BMC Plant Biology

Fig. 3

From: A cysteine-rich receptor-like protein kinase CaCKR5 modulates immune response against Ralstonia solanacearum infection in pepper

Fig. 3

Silencing of CaCRK5 in pepper enhanced susceptibility to the infection of R. solanacearum. a qRT-PCR analysis to check the expression of CaCRK5 in CaCRK5-silenced (TRV:CaCRK5) and empty vector control (TRV:00) pepper plants. Error bars represent the mean ± SD of three independent replicates. b Phenotypes of control and VIGS pepper plants. After the VIGS was established, 4-week-old plants were root-inoculated with R. solanacearum. Photographs were taken at 10 d post R. solanacearum infection. c Rate of disease index on a scale of 0 to 4 in CaCRK5-silenced and control plants. d Growth of R. solanacearum in leaves of in CaCRK5 silenced and control plants at 3 d post leaf-inoculation of R. solanacearum. The data are shown as means ± SD from three independent repeats (n = 3, *, P < 0.05, Mann-Whitney U test). e Visualization of diaminobenzidine (DAB) and trypan blue staining 48 h post leaf-inoculation with R. solanacearum. f The expression levels of defense-related genes in CaCRK5-silenced and control plants. Asterisks indicate statistically significant differences (Mann-Whitney U test,*P < 0.05)

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