Fig. 4

Prediction of infection-responsive microRNAs from the B. napus genome. A Histogram of the 73 conserved miRNA families. The y-axis shows the identified conserved miRNA family name and the x-axis shows the number of sequences (isomiRs) identified for each miRNA family; red bars are the significantly differentially expressed miRNA families. An isomiR is one of a family of highly similar miRNA sequences derived from either the guide or passenger strand. Green miRNA families were not differentially expressed. Those in other colours contained differentially expressed miRNAs and the colours correspond with D. B Histogram of read sizes from 529 conserved miRNAs. The y axis depicts the percentage of reads and the x axis read length in nucleotides. C Histogram of the 529 miRNAs showing percentage of reads (y axis) of each size class (x axis) that had each of the four nucleotides (AGCU) in their 5′ position. D 20 differentially expressed miRNAs with log₂(fold change) on the y axis. Colours correspond with miRNA families in A