Fig. 1

TGA7 regulates flowering time in Arabidopsis. a The structure of the TGA7 coding region. Black boxes, gray boxes, and black lines represent exons, untranslated regions, and introns, respectively. The point mutation is shown underneath. Red arrowheads indicate the positions of primers used in b. b The cropped gels of the CAPS analysis of the wild-type and tga7 mutant. Genomic DNAs of the wild-type and tga7 mutant were amplified using the CAPS markers list in Additional file 1, and then, the PCR products were digested with EcoRV. ctga7 showed a delayed-flowering phenotype under LD conditions. Scale bar = 2 cm. d and e Flowering times of tga7 grown under LD (d) and SD (e) conditions. Values are from at least 10 plants showing specific genotypes. Asterisks indicate significant differences in flowering time between the WT and tga7 mutant (Student’s t test, p ≤ 0.05). f The expression level of TGA7 in various tissues of WT plants was analyzed by qRT-PCR (n = 3, ±standard deviations). JRL, juvenile rosette leaves; Rt, roots; ARL, adult rosette leaves; CL, cauline leaves; FL, flowers; St, inflorescence stems; Sil, siliques. gtga7 35 S:TGA7 exhibited a flowering time comparable to that of WT plants under LD conditions. Scale bar = 2 cm. h Flowering time of tga7 35 S:TGA7 grown under LD conditions. Values are representative of at least 15 plants showing specific genotypes. Asterisks indicate significant differences between the WT and tga7 mutant in flowering time (Student’s t test, p ≤ 0.05). i The TGA7 expression levels in independent TGA7-overexpression lines at 9 DAG. Error bars denote standard deviations