Fig. 2

Clustering analysis of differentially expressed genes (DEGs) in the shoots and roots of rapeseed plants under varying Cd abundances. (A) Heat maps showing the differential expression fold-changes that were identified by RNA-seq and RT-qPCR assays. (B) Consistency analysis of gene expression levels between RNA-seq and RT-qPCR assays. (C) Clustering trees among the three biological replicates of each sample. Mock means the Cd-free condition. H_Cd, high Cd; L_Cd, low Cd; S, shoot; R, root. (D) Principal component analysis of genome-wide differential gene expression profiling in the shoots and roots of rapeseed plants under high Cd and low Cd conditions. (E-F) Venn diagram showing DEGs in the shoots (E) and roots (F) of rapeseed plants under high Cd and low Cd conditions. (G, H) Venn diagram showing the upregulated and downregulated DEGs in the shoots (G) and roots (H) of rapeseed plants under high Cd and low Cd conditions