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Fig. 6 | BMC Plant Biology

Fig. 6

From: The core autophagy machinery is not required for chloroplast singlet oxygen-mediated cell death in the Arabidopsis thaliana plastid ferrochelatase two mutant

Fig. 6

ATG-dependent autophagy is not required for selective chloroplast degradation in fc2 mutants. Chloroplast ultrastructure was assessed by transmission electron microscopy (TEM) in the atg mutants. Shown are representative A intact and B degrading chloroplasts in four day old seedlings grown under 24 h constant light conditions. Arrows indicate unusual horseshoe membrane structures. Shown is a blebbing chloroplast interacting with the central vacuole in the C fc2-1 atg5 and D fc2-1 atg7 mutants. Arrows indicate the bleb-like structure protruding into the central vacuole. The right panel is a zoomed-in image of the boxed region. b; bleb, c; cytoplasm, cv; central vacuole, m; mitochondria, p; plastid. Bars = 1 µm. E Mean chloroplast area (± SEM) from the same seedlings (n ≥ 10). Statistical analyses were performed using one-way ANOVA tests followed by Tukey's HSD. Different letters above bars indicate significant differences (p value ≤ 0.05). Separate statistical analyses were performed for seedlings in the wt and fc2-1 backgrounds and the significance for the fc2-1 background group is denoted by letters with a ʹ. Closed circles represent individual data points

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