Skip to main content
Fig. 4 | BMC Plant Biology

Fig. 4

From: The core autophagy machinery is not required for chloroplast singlet oxygen-mediated cell death in the Arabidopsis thaliana plastid ferrochelatase two mutant

Fig. 4

Blocking ATG-dependent autophagy does not suppress 1O2-induced cell death in fc2 adult plants. The phenotypes of fc2-1 atg double mutant plants were assessed in the adult stage. A Three-week old plants grown in 24 h constant light or under stressed conditions (two weeks in 24 h constant light and one week in 16 h light/8 h dark cycling light conditions). B Mean biomass (± SD) of same plants (n = 8 plants). C Representative trypan blue stains of single leaves from same plants. Dark blue color is indicative of cell death. D Quantification of mean trypan blue signal (± SEM) from plants in 16 h light/8 h dark cycling light conditions (n ≥ 6 leaves from individual plants). Statistical analyses were performed by one-way ANOVA tests followed by Tukey's HSD. Different letters above bars indicate significant differences (p value ≤ 0.05). For panel B, separate statistical analyses were performed for the different light treatments and the significance for the light stressed group is denoted by letters with a ʹ. In all bar graphs, closed circles represent individual data points

Back to article page