Fig. 3

Blocking ATG-dependent autophagy does not suppress ¹O₂-induced cell death in fc2 seedlings. atg mutations were tested for their ability to suppress cell death in the fc2-1 mutant. A Seven-day old seedlings grown in constant light (24 h) or 6 h light/18 h dark (6 h) cycling light conditions. B Trypan blue stains of seedlings from panel A. The dark blue color is indicative of cell death. C Mean values (± SEM) of the trypan blue signal in panel B (n ≥ 10 seedlings). D Mean chlorophyll content (µg/seedling) (± SEM) of groups of six-day old seedlings grown in 24 h light or 6 h cycling light conditions (n = 3 biological replicates)(y-axis in log₂ scale). E Representative images of maximum quantum yield of PSII (F_v/F_m) measured from three-day-old seedlings grown in the indicated light regiment. F Changes in F_v/F_m of seedlings grown over seven days in 6 h cycling light conditions. Mean values are shown ± SEM (n = 3 biological replicates). Statistical analyses were performed by one-way ANOVA tests followed by Tukey's HSD. Different letters above bars indicate significant differences (p value ≤ 0.05). For panel D, separate statistical analyses were performed for the different light treatments and the significance for the 6 h cycling values is denoted by letters with a ʹ. In all bar graphs, closed circles represent individual data points