Fig. 5
Root high-affinity and low-affinity 15NO3– uptake assay under nitrogen starvation and nitrate induction. A Seedlings were treated with nitrogen starvation (-N) for 2 days. Seedlings grown in normal hydroponic medium were used as the control; B Nitrogen-starved seedlings were then resupplied with 5 mM KNO3 for 2 h. Nitrogen-starved seedlings resupplied with 5 mM KCl were used as the control. The root high-affinity and low-affinity 15NO3– uptake abilities were detected in 0.1 mM or 5 mM K15NO3 solution, respectively, for 5 min. Data shown as mean ± s.d. of three independent biological replicates, ** p < 0.01