|
Primer
|
Sequence 5´to 3´
|
T/°C Annealing temperature
|
SR
|
NT
|
NP
|
|---|
|
cpDNA
|
|
trnL intron
|
F: CGAAATCGGTAGACGCTACG
|
48
| | | |
|
R: GGGGATAGAGGGACTTGAAC
| | | | |
|
psbJ-petA
|
F: ATAGGTACTGTAR*CYGGTATT
|
52
| | | |
|
R: AACAR*TTY*GAR*AAGGTTCAATT
| | | | |
|
ISSR
|
|
807
|
(AG)8 T
|
52
|
230–1500
|
21
|
20
|
|
808
|
(AG)8C
|
52
|
180–1700
|
20
|
19
|
|
810
|
(GA)8 T
|
52
|
210–1520
|
22
|
22
|
|
835
|
(AG)8Y*C
|
58
|
170–1700
|
19
|
17
|
|
836
|
(AG)8Y*A
|
50
|
250–1700
|
20
|
20
|
|
840
|
(GA)8Y*T
|
52
|
200–1500
|
22
|
22
|
|
841
|
(GA)8Y*C
|
52
|
180–1780
|
23
|
22
|
|
847
|
(CA)8R*C
|
52
|
280–1700
|
24
|
24
|
|
859
|
(TG)8RC
|
49
|
290–1700
|
24
|
24
|
|
887
|
DVD*(TC)7
|
52
|
220–1700
|
23
|
23
|
|
Total
|
-
|
-
|
-
|
218
|
213
|
- *Y: C/G, R: A/T, D: A/G/T; SR Size range of amplified fragments, NT Number of total bands, NP Number of polymorphic bands
