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Table 2 Attributes of two cpDNA and ten ISSR primers used in the present study

From: Population genetic structure and connectivity of a riparian selfing herb Caulokaempferia coenobialis at a fine-scale geographic level in subtropical monsoon forest

Primer

Sequence 5´to 3´

T/°C Annealing temperature

SR

NT

NP

cpDNA

 trnL intron

F: CGAAATCGGTAGACGCTACG

48

   

R: GGGGATAGAGGGACTTGAAC

    

 psbJ-petA

F: ATAGGTACTGTAR*CYGGTATT

52

   

R: AACAR*TTY*GAR*AAGGTTCAATT

    

ISSR

 807

(AG)8 T

52

230–1500

21

20

 808

(AG)8C

52

180–1700

20

19

 810

(GA)8 T

52

210–1520

22

22

 835

(AG)8Y*C

58

170–1700

19

17

 836

(AG)8Y*A

50

250–1700

20

20

 840

(GA)8Y*T

52

200–1500

22

22

 841

(GA)8Y*C

52

180–1780

23

22

 847

(CA)8R*C

52

280–1700

24

24

 859

(TG)8RC

49

290–1700

24

24

 887

DVD*(TC)7

52

220–1700

23

23

Total

-

-

-

218

213

  1. *Y: C/G, R: A/T, D: A/G/T; SR Size range of amplified fragments, NT Number of total bands, NP Number of polymorphic bands