Fig. 4

Respiratory activity of A. thaliana seedlings after treatment with priming compounds with and without flg22 elicitation, and reference cultivation without treatment with chemicals. OTR as a function of time of A. thaliana seedlings primed with 100 μM SA and 50 pM flg22 (red) or 25 µM Tyr020 and 50 pM flg22 (blue). Reference cultivations: without additives, negative control (black), addition of 100 μM SA only (red dotted line), 25 µM Tyr020 (blue dotted line), and with the addition of 50 pM flg22 only (green line). SA and Tyr020 were added at the -28 h time point relative to the time point of flg22 addition (0 h). Three-week-old A. thaliana seedlings were transferred to the µRAMOS device, according to Fig. 2D, and cultivated at 600 rpm shaking frequency, 3 mm shaking diameter, filling volume 1.5 mL, 20 °C, in MS medium with 20 g/L sucrose, initial pH 5.7. The number of replica (n) is specified for each condition in the legend. The standard deviation of replicates is indicated by the shadow surrounding the data