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Fig. 2 | BMC Plant Biology

Fig. 2

From: An illuminated respiratory activity monitoring system identifies priming-active compounds in plant seedlings

Fig. 2

Scheme of the setup for PrimAC identification using A. thaliana seedlings in the LED-µRAMOS device. A Seeds were sterilized by washing with 70% (v/v) ethanol and ethanol absolute (EtOH abs.). B A seed dispenser was used to transfer sterilized seeds to the wells of a MTP. C Cultivation in MTPs for the effective and synchronized germination of seeds. A. thaliana was grown on 1.5 mL MS medium for 3 weeks at a 16-h day/8-h night cycle. D The respiratory activity was measured in the absence of light at 20 °C and 600 rpm shaking frequency with a shaking diameter of 3 mm. Treatments with compounds was done 28 h before the OTR measurement were started. Seedlings were elicited using 50 pM flg22 at time zero (0 h)

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