Fig. 3
From: Stablization of ACOs by NatB mediated N-terminal acetylation is required for ethylene homeostasis
NTA mediated by NatB stabilizes ACOs. (A) N-terminal peptide of so far 5 known functional ACOs in ethylene biosynthesis in Arabidopsis, specific amino acid of NatB substrate signatures are shown in red colour. (B) Protein levels of ACO2, ACO3 and ACO4 extracted from the whole proteome analysis data. (C) GUS straining of proNBC-1::NBC-1-GUS/Col transgenic seedlings, bar = 2 mm. (D) Quantification results of ethylene contents. (E) ACO2 protein of dark-grown seedlings was detected using ACO2 antibody by WB test, the relative intensity of band in Col-7 was set to 1.0. (F) Transcript levels of ACO2 in dark-grown Col-7 and ckrc3 nbc-1 seedlings by RT-qPCR. “*”,“**” and “***” indicate significant differences according to ANOVA followed by Duncan’s multiple comparison, p < 0.05, p < 0.01 and p < 0.001, respectively