Fig. 6

Heatmaps of defence-related differentially expressed genes (DEG) across time points and tissues. Each figure shows the defence-related genes differentially up-regulated (log2 fold change in red) or down-regulated (in green) in wheat inoculated with Claviceps purpurea, relative to Mock-inoculated wheat, in stigma (S), transmitting (T) and base (B) tissues of the wheat ovary, at timepoints after inoculation: 24 h (24H), 48 h (48H), 72 h (72H), 5 days (5D) and 7 days (7D). DEG are defined by functional categories. a NBS-LRR class proteins (functional categories from top to bottom: RGA1, RGA2, RGA3, RPM1, RPP13, RPP8, RPS2, NBS-LRR); b Receptor protein kinases ()functional categories from top to bottom: CBL-interacting protein kinases (CIPK), Cysteine-rich receptor-like kinases (CRKs), Flagellin-sensing 2 (FLS2), GTPase activating 1, Lectin receptor kinases (LecRK), Mitogen-activated kinase (MAPK), serine/threonine kinases (STKs)); c Antifungal proteins ((functional categories from top to bottom: Bowman-Birk type trypsin inhibitor (BBI), beta purothionins, chitin elicitor-binding, chitinase, Cytochrome P450, Defensins, Glycine-rich proteins (GRPs), non-specific lipid transfer proteins (nsLTPs), polygalacturonase inhibiting protein (PGIP), plant-pathogenesis proteins (PPP)); d Endocytosis/Exocytosis related proteins; e Transcription factors; and f Programmed cell death related genes ((functional categories from top to bottom: Accelerated Cell Death 11 (ACD11), hexokinase (HXK), Harpin induced protein (HIN1), metacaspase, polyamine oxidase (PAO), polyphenol oxidase (PPO), Potassium transporter (PT), subtilisin-like proteases (SLP))