Fig. 7

Overexpression of ZmSnRK2.10 leads to delayed flowering time in Arabidopsis. (A) Flowering phenotype of the WT, Atsnrk2.2/2.3, OEZmSnRK2.10/WT, and OEZmSnRK2.10/Atsnrk2.2/2.3. The photograph was taken 35 days after growth under LD conditions. (B) Flowering time of the WT, Atsnrk2.2/2.3, OEZmSnRK2.10/WT, and OEZmSnRK2.10/Atsnrk2.2/2.3 under LD conditions was scored based on the days from germination to bolting. (C) Flowering time of the WT, Atsnrk2.2/2.3, OEZmSnRK2.10/WT, and OEZmSnRK2.10/Atsnrk2.2/2.3 under LD conditions was scored based on the number of rosette leaves at flowering stage. (D) qRT-PCR analysis of FLC gene in the WT, Atsnrk2.2/2.3, OEZmSnRK2.10/WT, and OEZmSnRK2.10/Atsnrk2.2/2.3. (E) qRT-PCR analysis of FT gene in the WT, Atsnrk2.2/2.3, OEZmSnRK2.10/WT, and OEZmSnRK2.10/Atsnrk2.2/2.3. Error bars represent SE from three biological replicates